ReferenceID 1731
Daphnetin inhibits corneal inflammation and neovascularization on a mouse model of corneal alkali burn
Int Immunopharmacol
Alkali burn is a significant contributor to corneal injury. Alkali burn-induced corneal inflammation often causes vision loss due to corneal neovascularization. Daphnetin (DAP) has been studied for its anti-inflammatory
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 1731
- Evidence Id
- 18321
- Core Evidence Id
- 18321
- Source Reference Id
- 3442
- Herb2 Reference Id
- HBREF004239
- Subject Paper Key
- HBIN022677_34920334
- Pubmed Id
- 34920334
- Doi
- 10.1016/j.intimp.2021.108434
- Paper Title
- Daphnetin inhibits corneal inflammation and neovascularization on a mouse model of corneal alkali burn
- Paper Abstract
- Alkali burn is a significant contributor to corneal injury. Alkali burn-induced corneal inflammation often causes vision loss due to corneal neovascularization. Daphnetin (DAP) has been studied for its anti-inflammatory and antiangiogenic properties with encouraging results. Driven by those encouraging results, we sought to explore the effects of DAP in treating alkali burn-induced corneal inflammation and neovascularization and its mechanism of action. We found that the angiogenesis processes of human umbilical vein endothelial cells (HUVECs) induced by vascular endothelial growth factor A (VEGF-A) were primarily attenuated by treatment with DAP, including proliferation, migration, and tube formation. Treatment of DAP significantly suppressed the VEGF-A-induced protein expression of VEGF receptor2 (VEGFR2), as well as the activation of downstream signal transducer and activator of transcription 3 (STAT3), AKT, and extracellular signal-regulated kinase (ERK) signaling. In the mouse corneal alkali burn model, the inflammatory cell infiltrations and neovascularization in the cornea caused by alkali burn were inhibited by 10 µM DAP eye drops. Alkali burn-induced corneal protein expression of VEGF-A, VEGFR2, phosphorylated (p-)STAT3, p-AKT, and p-ERK in corneal tissue were reduced mainly by DAP. Moreover, the upregulation of inflammatory caused by alkali burn in the pathological process was significantly neutralized by DAP. Mechanistically, the inflammatory response could be alleviated by DAP in the way of inhibiting the expression levels of TLR4, p-NF-κB, NLRP3, ASC, Cleaved-caspase-1 (p20), mature-IL-1β (p17), and N-GSDM. In conclusion, our findings confirmed that the corneal inflammation and neovascularization caused by alkali burn could be inhibited by DAP in vitro and in vivo, elucidating the underlying mechanisms of its protective effects. DAP may have tremendous therapeutic potential for the treatment of corneal alkali burn.
- Journal
- Int Immunopharmacol
- Publish Year
- 2021
- Experiment Subject
- mouse; human; huvecs
- Experiment Type
- Cell Experiment
- Phenotype Related
- Corneal Neovascularization; Alkali Burn-induced Corneal Inflammation; Corneal Inflammation; Neovascularization; Corneal Alkali Burn; Alkali Burn; Corneal Injury; Vision Loss
- Paper Title Cn
- Paper Title En
- Daphnetin inhibits corneal inflammation and neovascularization on a mouse model of corneal alkali burn
- Bilingual Status
- semi_complete