ReferenceID 1728
Chondroprotective and antiarthritic effects of Daphnetin used in vitro and in vivo osteoarthritis models
Life Sci
AIMS: Daphnetin (DAP) is a traditional Chinese drug usually used to treat cardiovascular diseases. Studies have confirmed the anti-inflammatory, antioxidant, anti-bacterial and insecticidal, anti-tumor and neuro-protecti
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- Reference Id
- 1728
- Evidence Id
- 18318
- Core Evidence Id
- 18318
- Source Reference Id
- 3438
- Herb2 Reference Id
- HBREF004235
- Subject Paper Key
- HBIN022677_31521691
- Pubmed Id
- 31521691
- Doi
- 10.1016/j.lfs.2019.116857
- Paper Title
- Chondroprotective and antiarthritic effects of Daphnetin used in vitro and in vivo osteoarthritis models
- Paper Abstract
- AIMS: Daphnetin (DAP) is a traditional Chinese drug usually used to treat cardiovascular diseases. Studies have confirmed the anti-inflammatory, antioxidant, anti-bacterial and insecticidal, anti-tumor and neuro-protective effects of DAP. However, its anti-arthritic potential remains unexplored. The aim of this study is to investigate the in vitro and in vivo chondroprotective effects of DAP. MAIN METHODS: The effect of DAP on primary rabbit chondrocytes was examined using recombinant human IL-1beta for 24 h. For the in vivo studies, rabbits were randomly divided into groups: a normal control group and osteoarthritis (OA) groups. The OA groups received three different doses of DAP for 4 or 8 weeks. The anti-arthritic effect of DAP was assessed using histopathological examinations, qRT-PCR, western blotting and immunohistochemical analysis. KEY FINDINGS: Both in vitro and in vivo results indicate that DAP exerts a protective effect against IL-1beta in chondrocytes. In vitro, DAP inhibits the expression of IL-6, IL-12, MMP-3, MMP-9 and MMP-13, induced by IL-1beta in rabbit chondrocytes, and stimulates the production of IL-10. The inhibitory effect of DAP on the MMPs is partially regulated by the inhibition of the PI3K/AKT, MAPK and NF-kappaB signaling pathways. The effect of DAP on OA may be attributed to the suppression of inflammatory factor secretion, chondrocyte apoptosis observed by the decrease in pro-apoptotic Caspase-3 and BAX, and the activation of anti-apoptotic BCL-2. SIGNIFICANCE: DAP has a broad range of prospects in the treatment of OA, which provides a novel therapeutic strategy for OA.
- Journal
- Life Sci
- Publish Year
- 2020
- Experiment Subject
- human; rabbit
- Experiment Type
- Animal Experiment
- Phenotype Related
- Osteoarthritis; Cardiovascular Diseases
- Paper Title Cn
- Paper Title En
- Chondroprotective and antiarthritic effects of Daphnetin used in vitro and in vivo osteoarthritis models
- Bilingual Status
- semi_complete