ReferenceID 1726
Danshensu inhibits the IL-1β-induced inflammatory response in chondrocytes and osteoarthritis possibly via suppressing NF-κB signaling pathway
Mol Med
PURPOSE: Osteoarthritis (OA) is the most common inflammatory disease associated with pain and cartilage destruction. Interleukin (IL)-1beta is widely used to induce inflammatory response in OA models. This study aimed to
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- Reference Id
- 1726
- Evidence Id
- 18316
- Core Evidence Id
- 18316
- Source Reference Id
- 3436
- Herb2 Reference Id
- HBREF004233
- Subject Paper Key
- HBIN022650_34284715
- Pubmed Id
- 34284715
- Doi
- 10.1186/s10020-021-00329-9
- Paper Title
- Danshensu inhibits the IL-1β-induced inflammatory response in chondrocytes and osteoarthritis possibly via suppressing NF-κB signaling pathway
- Paper Abstract
- PURPOSE: Osteoarthritis (OA) is the most common inflammatory disease associated with pain and cartilage destruction. Interleukin (IL)-1beta is widely used to induce inflammatory response in OA models. This study aimed to explore the role of Danshensu (DSS) in IL-1beta-induced inflammatory responses in OA. METHODS: IL-1beta was used to induce chondrocyte inflammation. Cell viability was evaluated by Cell Counting Kit-8 (CCK-8) assay. IL-6, COX-2, TNF-alpha, and iNOS mRNA levels were detected by qRT-PCR. MMP3, MMP13, ADAMTS4, ADAMTS5, Aggrecan, Collagen, p-IkappaBalpha, and p-p65 protein levels were detected by Western blot. An OA mouse model was established by surgical destabilization of the medial meniscus (DMM), and the Osteoarthritis Research Society International (OARSI) score was evaluated by H&E staining. RESULTS: DSS did not affect the levels of inflammatory indicators including IL-6, COX-2, TNF-alpha, iNOS, PEG2, and NO but suppressed COX-2 and iNOS protein expression in IL-1beta treated chondrocytes. In addition, DSS downregulated IL-1beta-enhanced expression of MMP3, MMP13, ADAMTS4, and ADAMTS5 and upregulated aggrecan and collagen expression. Moreover, DSS significantly inhibited IL-1beta-induced phosphorylation of p-IkappaBalpha and p-p65 in a dose-dependent manner in chondrocytes, suggesting it plays a role in the NF-kappaB signaling pathway. Furthermore, DSS significantly reduced DMM-induced cartilage OARSI score in mice, further demonstrating its protective role in OA progression in vivo. CONCLUSIONS: Our study revealed the protective role of DSS in OA, suggesting that DSS might act as a potential treatment for OA.
- Journal
- Mol Med
- Publish Year
- 2021
- Experiment Subject
- mouse; il-1beta treated chondrocytes
- Experiment Type
- Animal Experiment
- Phenotype Related
- Cartilage Destruction; Pain; Chondrocyte Inflammation; Inflammatory Disease; Osteoarthritis
- Paper Title Cn
- Paper Title En
- Danshensu inhibits the IL-1β-induced inflammatory response in chondrocytes and osteoarthritis possibly via suppressing NF-κB signaling pathway
- Bilingual Status
- semi_complete