ReferenceID 1717
Cytisine exerts anti-tumour effects on lung cancer cells by modulating reactive oxygen species-mediated signalling pathways
Artif Cells Nanomed Biotechnol
Cytisine is a natural product isolated from plants and is a member of the quinolizidine alkaloid family. This study aims to investigate the effect of cytisine in human lung cancer. Cell viability was determined using the
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- Reference Id
- 1717
- Evidence Id
- 18307
- Core Evidence Id
- 18307
- Source Reference Id
- 3422
- Herb2 Reference Id
- HBREF004219
- Subject Paper Key
- HBIN022522_31852250
- Pubmed Id
- 31852250
- Doi
- 10.1080/21691401.2019.1699813
- Paper Title
- Cytisine exerts anti-tumour effects on lung cancer cells by modulating reactive oxygen species-mediated signalling pathways
- Paper Abstract
- Cytisine is a natural product isolated from plants and is a member of the quinolizidine alkaloid family. This study aims to investigate the effect of cytisine in human lung cancer. Cell viability was determined using the CCK-8 assay, and the results showed that cytisine inhibited the growth of lung cancer cell lines. The apoptotic effects were evaluated using flow cytometry, and the results showed that cytisine induced mitochondrial-dependent apoptosis through loss of the mitochondrial membrane potential; increased expression of BAD, cleaved caspase-3, and cleaved-PARP; and decreased expression levels of Bcl-2, pro-caspase-3, and pro-PARP. In addition, cytisine caused G2/M phase cell cycle arrest that was associated with inhibiting the AKT signalling pathway. During apoptosis, cytisine increased the phosphorylation levels of JNK, p38, and I-kappaB, and decreased the phosphorylation levels of ERK, STAT3, and NF-kappaB. Furthermore, cytisine treatment led to the generation of ROS, and the NAC attenuated cytisine-induced apoptosis. In vivo, cytisine administration significantly inhibited the lung cancer cell xenograft tumorigenesis. In conclusion, cytisine plays a critical role in suppressing the carcinogenesis of lung cancer cells through cell cycle arrest and induction of mitochondria-mediated apoptosis, suggesting that it may be a promising candidate for the treatment of human lung cancer.
- Journal
- Artif Cells Nanomed Biotechnol
- Publish Year
- 2020
- Experiment Subject
- human; lung cancer cell lines
- Experiment Type
- Cell Experiment
- Phenotype Related
- Lung Cancer
- Paper Title Cn
- Paper Title En
- Cytisine exerts anti-tumour effects on lung cancer cells by modulating reactive oxygen species-mediated signalling pathways
- Bilingual Status
- semi_complete