ReferenceID 1618
Cucurbitacin B Inhibits Cell Proliferation by Regulating X-Inactive Specific Transcript Expression in Tongue Cancer
Front Oncol
Cucurbitacin B (CuB), a natural product, has anti-tumor effects on various cancers. In order to investigate the expression of long non-coding RNAs (lncRNA), we carried out RNA sequencing (RNA-seq) and quantitative PCR (q
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- Reference Id
- 1618
- Evidence Id
- 18208
- Core Evidence Id
- 18208
- Source Reference Id
- 3244
- Herb2 Reference Id
- HBREF004041
- Subject Paper Key
- HBIN021845_34295808
- Pubmed Id
- 34295808
- Doi
- 10.3389/fonc.2021.651648
- Paper Title
- Cucurbitacin B Inhibits Cell Proliferation by Regulating X-Inactive Specific Transcript Expression in Tongue Cancer
- Paper Abstract
- Cucurbitacin B (CuB), a natural product, has anti-tumor effects on various cancers. In order to investigate the expression of long non-coding RNAs (lncRNA), we carried out RNA sequencing (RNA-seq) and quantitative PCR (qPCR). The data indicated that CAL27 and SCC9 tongue squamous cell carcinoma (TSCC) cells had reduced expression of X-inactive specific transcript (XIST) after CuB treatment. Moreover, our results showed increased expression of XIST in human tongue cancer. In this study, CuB treatment inhibited proliferation, migration and invasion of SCC9 cells, and induced cellular apoptosis. Interestingly, knockdown of XIST led to inhibition of cell proliferation and induced apoptosis in vitro. In addition, reduced expression of XIST suppressed cell migration and invasion. MicroRNA 29b (miR-29b) was identified as a direct target of XIST. Previous reports indicated that miR-29b regulates p53 protein. Our results suggest that increased expression of miR-29b induces cell apoptosis through p53 protein. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system validated the role of XIST knockout in tumor development in vivo. Together, these results suggest that CuB exerts significant anti-cancer activity by regulating expression of XIST via miR-29b.
- Journal
- Front Oncol
- Publish Year
- 2021
- Experiment Subject
- human; scc9 cells; scc9 tongue squamous cell carcinoma (tscc) cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cancers; Tumor; Tongue Cancer; Scc9 Tongue Squamous Cell Carcinoma
- Paper Title Cn
- Paper Title En
- Cucurbitacin B Inhibits Cell Proliferation by Regulating X-Inactive Specific Transcript Expression in Tongue Cancer
- Bilingual Status
- semi_complete