ReferenceID 1603
Crocetin Protected Human Hepatocyte LO2 Cell From TGF-β-Induced Oxygen Stress and Apoptosis but Promoted Proliferation and Autophagy via AMPK/m-TOR Pathway
Front Public Health
Objective: To investigate the protective effects of crocetin against transforming growth factor-β (TGF-β)-induced injury in LO2 cells. Methods: Human hepatocyte LO2 cells were pre-treated with crocetin (10 μM) for 6, 12,
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Record Fields
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- Reference Id
- 1603
- Evidence Id
- 18193
- Core Evidence Id
- 18193
- Source Reference Id
- 3210
- Herb2 Reference Id
- HBREF004007
- Subject Paper Key
- HBIN021703_35836988
- Pubmed Id
- 35836988
- Doi
- 10.3389/fpubh.2022.909125
- Paper Title
- Crocetin Protected Human Hepatocyte LO2 Cell From TGF-β-Induced Oxygen Stress and Apoptosis but Promoted Proliferation and Autophagy via AMPK/m-TOR Pathway
- Paper Abstract
- Objective: To investigate the protective effects of crocetin against transforming growth factor-β (TGF-β)-induced injury in LO2 cells. Methods: Human hepatocyte LO2 cells were pre-treated with crocetin (10 μM) for 6, 12, and 24 h, and then induced by TGF-β. Proliferation, oxidative stress, apoptosis, autophagy, and related proteins were assessed. Results: Crocetin pre-treating promoted proliferation but suppressed apoptosis in TGF-β-induced LO2 cells. Crocetin protected LO2 cells from TGF-β-induced inflammation and oxygen stress by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) but enhancing superoxide dismutase (SOD) and glutathione (GSH). Autophagy was suppressed in TGF-β but crocetin promoted autophagy in LO2 cells by mediating Adenosine 5'-monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (m-TOR) signaling pathway via upregulating p-AMPK and p-Beclin-1 but downregulating p-mTOR. Conclusions: Crocetin protected LO2 cells from TGF-β-induced damage by promoting proliferation and autophagy, and suppressing apoptosis and anti-inflammation via regulation of AMPK/m-TOR signaling pathway.
- Journal
- Front Public Health
- Publish Year
- 2022
- Experiment Subject
- human; human hepatocyte lo2 cells; lo2 cells; tgf-β-induced lo2 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Crocetin Protected Human Hepatocyte LO2 Cell From TGF-β-Induced Oxygen Stress and Apoptosis but Promoted Proliferation and Autophagy via AMPK/m-TOR Pathway
- Bilingual Status
- semi_complete