ReferenceID 1556
Citrulline protects human retinal pigment epithelium from hydrogen peroxide and iron/ascorbate induced damages
J Cell Mol Med
Oxidative stress plays an important role in the ageing of the retina and in the pathogenesis of retinal diseases such as age-related macular degeneration (ARMD). Hydrogen peroxide is a reactive oxygen species generated b
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Record Fields
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- Reference Id
- 1556
- Evidence Id
- 18146
- Core Evidence Id
- 18146
- Source Reference Id
- 3108
- Herb2 Reference Id
- HBREF003905
- Subject Paper Key
- HBIN021006_35460170
- Pubmed Id
- 35460170
- Doi
- 10.1111/jcmm.17294
- Paper Title
- Citrulline protects human retinal pigment epithelium from hydrogen peroxide and iron/ascorbate induced damages
- Paper Abstract
- Oxidative stress plays an important role in the ageing of the retina and in the pathogenesis of retinal diseases such as age-related macular degeneration (ARMD). Hydrogen peroxide is a reactive oxygen species generated by the photo-excited lipofuscin that accumulates during ageing in the retinal pigment epithelium (RPE), and the age-related accumulation of lipofuscin is associated with ARMD. Iron also accumulates with age in the RPE that may contribute to ARMD as an important source of oxidative stress. The aim of this work was to investigate the effects of L-Citrulline (CIT), a naturally occurring amino acid with known antioxidant properties, on oxidative stressed cultured RPE cells. Human RPE (ARPE-19) cells were exposed to hydrogen peroxide (H 2 O 2 ) or iron/ascorbate (I/A) for 4 h, either in the presence of CIT or after 24 h of pretreatment. Here, we show that supplementation with CIT protects ARPE-19 cells against H 2 O 2 and I/A. CIT improves cell metabolic activity, decreases ROS production, limits lipid peroxidation, reduces cell death and attenuates IL-8 secretion. Our study evidences that CIT is able to protect human RPE cells from oxidative damage and suggests potential protective effect for the treatment of retinal diseases associated with oxidative stress.
- Journal
- J Cell Mol Med
- Publish Year
- 2022
- Experiment Subject
- human; arpe-19 cells; human rpe (arpe-19) cells; oxidative stressed cultured rpe cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Age-related Macular Degeneration; Retinal Diseases
- Paper Title Cn
- Paper Title En
- Citrulline protects human retinal pigment epithelium from hydrogen peroxide and iron/ascorbate induced damages
- Bilingual Status
- semi_complete