ReferenceID 1403
Camphene Attenuates Skeletal Muscle Atrophy by Regulating Oxidative Stress and Lipid Metabolism in Rats
Nutrients
Sarcopenia- or cachexia-related muscle atrophy is due to imbalanced energy metabolism and oxidative stress-induced muscle dysfunction. Monoterpenes play biological and pharmacological reactive oxygen species (ROS) scaven
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- Reference Id
- 1403
- Evidence Id
- 17993
- Core Evidence Id
- 17993
- Source Reference Id
- 2814
- Herb2 Reference Id
- HBREF003611
- Subject Paper Key
- HBIN019487_33287349
- Pubmed Id
- 33287349
- Doi
- 10.3390/nu12123731
- Paper Title
- Camphene Attenuates Skeletal Muscle Atrophy by Regulating Oxidative Stress and Lipid Metabolism in Rats
- Paper Abstract
- Sarcopenia- or cachexia-related muscle atrophy is due to imbalanced energy metabolism and oxidative stress-induced muscle dysfunction. Monoterpenes play biological and pharmacological reactive oxygen species (ROS) scavenging roles. Hence, we explored the effects of camphene, a bicyclic monoterpene, on skeletal muscle atrophy in vitro and in vivo. We treated L6 myoblast cells with camphene and then examined the ROS-related oxidative stress using Mito TrackerTM Red FM and anti-8-oxoguanine antibody staining. To investigate lipid metabolism, we performed real-time polymerase chain reactions, holotomographic microscopy, and respiratory gas analysis. Rat muscle atrophy in in vivo models was observed using 18F-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography and immunocytochemistry. Camphene reversed the aberrant cell size and muscle morphology of L6 myoblasts under starvation and in in vivo models. Camphene also attenuated E3 ubiquitin ligase muscle RING-finger protein-1, mitochondrial fission, and 8-oxoguanine nuclear expression in starved myotubes and hydrogen peroxide (H2O2)-treated cells. Moreover, camphene significantly regulated lipid metabolism in H2O2-treated cells and in vivo models. These findings suggest that camphene may potentially affect skeletal muscle atrophy by regulating oxidative stress and lipid metabolism.
- Journal
- Nutrients
- Publish Year
- 2020
- Experiment Subject
- rat; h2o2-treated cells; hydrogen peroxide (h2o2)-treated cells; l6 myoblast cells; l6 myoblasts
- Experiment Type
- Cell Experiment
- Phenotype Related
- Muscle Atrophy; Muscle Dysfunction; Sarcopenia- Or Cachexia-related Muscle Atrophy
- Paper Title Cn
- Paper Title En
- Camphene Attenuates Skeletal Muscle Atrophy by Regulating Oxidative Stress and Lipid Metabolism in Rats
- Bilingual Status
- semi_complete