ReferenceID 137
Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma
Cancer Lett
We investigated the use of cucurbitacin B, a plant-derived tetracyclic triterpenoid, as a single agent or in combination with methotrexate (MTX) for human osteosarcoma (OS) treatment. Cucurbitacin B showed antiproliferat
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 137
- Evidence Id
- 16727
- Core Evidence Id
- 16727
- Source Reference Id
- 237
- Herb2 Reference Id
- HBREF000446
- Subject Paper Key
- HBIN021845_21440986
- Pubmed Id
- 21440986
- Doi
- 10.1016/j.canlet.2011.03.001
- Paper Title
- Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma
- Paper Abstract
- We investigated the use of cucurbitacin B, a plant-derived tetracyclic triterpenoid, as a single agent or in combination with methotrexate (MTX) for human osteosarcoma (OS) treatment. Cucurbitacin B showed antiproliferative activity against seven human OS cell lines in vitro accompanying G2/M cell cycle arrest, apoptosis, and inhibition of ERK, Akt, and mTOR proteins. Cucurbitacin B in combination with MTX synergistically inhibited OS cell growth in vitro. Low-dose cucurbitacin B (LD-CuB, 0.5 mg/kg body weight) or low-dose MTX (LD-MTX, 150 mg/kg) failed to decrease the size of human OS xenografts in nude mice. However, combined therapy at identical concentrations inhibited tumor growth by 62% vs. LD-CuB and 81% vs. LD-MTX (p<0.001). Strikingly, the effect persisted even when the dose of MTX was decreased by two thirds (VLD-MTX, 50 mg/kg). In conclusion, cucurbitacin B alone or in combination with MTX shows promising antiproliferative activity against human OS.
- Journal
- Cancer Lett
- Publish Year
- 2011
- Experiment Subject
- human os cell lines,nude mice
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma
- Bilingual Status
- semi_complete