ReferenceID 1369

Pharmacological investigation of brucine anti-ulcer potential

Front Pharmacol

Gastric ulcer is one of the most common chronic gastrointestinal diseases characterized by a significant defect in the mucosal barrier. The current study has been conducted to evaluate the brucine anti-ulcer effect. Bruc

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Reference Id
1369
Evidence Id
17959
Core Evidence Id
17959
Source Reference Id
2754
Herb2 Reference Id
HBREF003551
Subject Paper Key
HBIN018923_36059979
Pubmed Id
36059979
Doi
10.3389/fphar.2022.886433
Paper Title
Pharmacological investigation of brucine anti-ulcer potential
Paper Abstract
Gastric ulcer is one of the most common chronic gastrointestinal diseases characterized by a significant defect in the mucosal barrier. The current study has been conducted to evaluate the brucine anti-ulcer effect. Brucine has binding energy values ranging from -2.99 to -8.11 kcal/mol against chosen targets, according to in silico research. Brucine exhibits an inhibitory effect against Helicobacter pylori . In vivo findings revealed that brucine (3 mg/kg) showed effective results in healing ethanol-induced ulcer lesions of the gastric region in rats. Brucine showed an inhibitory effect against H + /K + -ATPase. Levels of glutathione, glutathione-s-transferase, and catalase were enhanced in the gastric rat tissue with the use of brucine, while a significant decrease in lipid peroxide levels was seen. Histopathological evaluation showed improvement in cellular architecture and a decrease in inflammatory indicators like cyclooxygenase, tumor necrosis factor, and nuclear factor kappa B expression, validated through immunohistochemistry, enzyme-linked immunosorbent assay, and Western blot techniques. In the reverse transcription-polymerase chain reaction, brucine decreased H + /K + -ATPase mRNA levels. This study reveals that brucine possesses stable binding affinities against selected targets. Brucine exhibits an anti-ulcer effect, mediated via anti- H. pylori , H + /K + -ATPase inhibition, and antioxidant and anti-inflammatory pathways.
Journal
Front Pharmacol
Publish Year
2022
Experiment Subject
rat
Experiment Type
Animal Experiment
Phenotype Related
Chronic Gastrointestinal Diseases; Ethanol-induced Ulcer Lesions; Gastric Ulcer; Tumor
Paper Title Cn
Paper Title En
Pharmacological investigation of brucine anti-ulcer potential
Bilingual Status
semi_complete