ReferenceID 1352
Oxyberberine Prevented Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Mitophagy
Oxid Med Cell Longev
Acute lung injury (ALI) is a serious respiratory syndrome characterized with uncontrolled inflammatory response. Oxyberberine has strong potential for clinical usage since it showed strong anti-inflammatory, antifungal,
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Record Fields
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- Reference Id
- 1352
- Evidence Id
- 17942
- Core Evidence Id
- 17942
- Source Reference Id
- 2701
- Herb2 Reference Id
- HBREF003498
- Subject Paper Key
- HBIN017927_33728026
- Pubmed Id
- 33728026
- Doi
- 10.1155/2021/6675264
- Paper Title
- Oxyberberine Prevented Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Mitophagy
- Paper Abstract
- Acute lung injury (ALI) is a serious respiratory syndrome characterized with uncontrolled inflammatory response. Oxyberberine has strong potential for clinical usage since it showed strong anti-inflammatory, antifungal, and antiarrhythmic effects in various diseases. In the present study, we evaluated whether oxyberberine can inhibit lipopolysaccharide- (LPS-) induced ALI in vivo and further evaluated the possible involvement of mitophagy in vitro by using A549 cells, a human lung epithelial cell line. Our in vivo study shows that oxyberberine significantly inhibited LPS-induced lung pathological injury and lung edema, as indicated by the changes in lung wet/dry ratio and total protein levels in the BALF in mice. Moreover, oxyberberine inhibited inflammation, as indicated by the changes of neutrophil accumulation and production of proinflammatory cytokines including tumor necrosis factor alpha (TNF-alpha), interleukin 1beta (IL-1beta), and IL-6 in both the lung and bronchoalveolar lavage fluid (BALF) in ALI mice. Our in vitro study shows that LPS significantly decreased the protein level of mitochondrial proteins, including cytochrome c oxidase subunit IV (COX IV), p62, and mitofusin-2 (Mfn2) in A549 cells. In addition, LPS induced significant Parkin1 translocation from cytoplasm to mitochondria. These changes were significantly inhibited by oxyberberine. Notably, the inhibitory effect of oxyberberine was almost totally lost in the presence of lysosome fusion inhibitor bafilomycin A1 (Baf), a mitophagy inhibitor. In conclusion, the present study demonstrated that oxyberberine alleviated LPS-induced inflammation in ALI via inhibition of Parkin-mediated mitophagy.
- Journal
- Oxid Med Cell Longev
- Publish Year
- 2021
- Experiment Subject
- mouse; human; a549 cells; human lung epithelial cell line
- Experiment Type
- Cell Experiment
- Phenotype Related
- Acute Lung Injury; Tumor; Lung Edema; Respiratory Syndrome
- Paper Title Cn
- Paper Title En
- Oxyberberine Prevented Lipopolysaccharide-Induced Acute Lung Injury through Inhibition of Mitophagy
- Bilingual Status
- semi_complete