ReferenceID 1317
Identification of a pro-elongation effect of diallyl disulfide, a major organosulfur compound in garlic oil, on microglial process
J Nutr Biochem
Microglia are the innate immune cells in the nervous system. In the resting state, they display a ramified morphology, while upon disease stimulation their processes would be retracted, along with pro-inflammatory cytoki
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Record Fields
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- Reference Id
- 1317
- Evidence Id
- 17907
- Core Evidence Id
- 17907
- Source Reference Id
- 3501
- Herb2 Reference Id
- HBREF004298
- Subject Paper Key
- HBIN023556_32135404
- Pubmed Id
- 32135404
- Doi
- 10.1016/j.jnutbio.2019.108323
- Paper Title
- Identification of a pro-elongation effect of diallyl disulfide, a major organosulfur compound in garlic oil, on microglial process
- Paper Abstract
- Microglia are the innate immune cells in the nervous system. In the resting state, they display a ramified morphology, while upon disease stimulation their processes would be retracted, along with pro-inflammatory cytokine overproduction. Reversing microglial process retraction may help reduce pro-inflammatory cytokine production and restore microglia's ability to scan surrounding environments, rendering brain function regulation to be more effective. We found that diallyl disulfide (DADS), a major organosulfur compound in garlic oil, administered at different doses and time points, promoted microglial process elongation in both cultured systems and prefrontal cortexes in mice in a reversible manner. Lipopolysaccharide (LPS), a classical activator of microglia, did not affect this pro-elongation effect of DADS at conditions in vitro and in vivo. Functional studies revealed that DADS pre-treatment attenuated LPS-induced decreases in levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) mRNA as well as LPS-induced increases in levels of IL-10 and CD206 mRNA in both cultured microglia and prefrontal cortexes in mice. Protein kinase B (Akt) inhibition attenuated the pro-elongation effect of DADS on microglial process and blocked the regulatory effects of DADS on LPS-induced inflammatory responses in both cultured microglia and prefrontal cortexes in mice. In an in vivo model of neuroinflammation, DADS pre-treatment prevented LPS-induced retraction of microglial process in the prefrontal cortex in mice and attenuated LPS-induced increase in immobility time in the tail suspension test and forced swim test. These results indicate that DADS induces an Akt-dependent elongation of microglia process, along with the induction of an anti-inflammatory phenotype.
- Journal
- J Nutr Biochem
- Publish Year
- 2020
- Experiment Subject
- mouse; garlic
- Experiment Type
- Cell Experiment
- Phenotype Related
- Tumor
- Paper Title Cn
- Paper Title En
- Identification of a pro-elongation effect of diallyl disulfide, a major organosulfur compound in garlic oil, on microglial process
- Bilingual Status
- semi_complete