ReferenceID 1209
Arctigenin Suppressed Epithelial-Mesenchymal Transition Through Wnt3a/β-Catenin Pathway in PQ-Induced Pulmonary Fibrosis
Front Pharmacol
Arctigenin (ATG), a major bioactive substance of Fructus Arctii, counters renal fibrosis; however, whether it protects against paraquat (PQ)-induced lung fibrosis remains unknown. The present study was to determine the e
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Record Fields
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- Reference Id
- 1209
- Evidence Id
- 17799
- Core Evidence Id
- 17799
- Source Reference Id
- 2405
- Herb2 Reference Id
- HBREF003202
- Subject Paper Key
- HBIN016609_33390951
- Pubmed Id
- 33390951
- Doi
- 10.3389/fphar.2020.584098
- Paper Title
- Arctigenin Suppressed Epithelial-Mesenchymal Transition Through Wnt3a/β-Catenin Pathway in PQ-Induced Pulmonary Fibrosis
- Paper Abstract
- Arctigenin (ATG), a major bioactive substance of Fructus Arctii, counters renal fibrosis; however, whether it protects against paraquat (PQ)-induced lung fibrosis remains unknown. The present study was to determine the effect of ATG on PQ-induced lung fibrosis in a mouse model and the underlying mechanism. Firstly, we found that ATG suppressed PQ-induced pulmonary fibrosis by blocking the epithelial-mesenchymal transition (EMT). ATG reduced the expressions of Vimentin and alpha-SMA (lung fibrosis markers) induced by PQ and restored the expressions of E-cadherin and Occludin (two epithelial markers) in vivo and in vitro. Besides, the Wnt3a/beta-catenin signaling pathway was significantly activated in PQ induced pulmonary fibrosis. Further analysis showed that pretreatment of ATG profoundly abrogated PQ-induced EMT-like phenotypes and behaviors in A549 cells. The Wnt3a/beta-catenin signaling pathway was repressed by ATG treatment. The overexpression of Wnt3a could weaken the therapeutic effect of ATG in A549 cells. These findings suggested that ATG could serve as a new therapeutic candidate to inhibit or even reverse EMT-like changes in alveolar type II cells during PQ-induced lung fibrosis, and unraveled that the Wnt3a/beta-catenin pathway might be a mechanistic tool for ATG to control pulmonary fibrosis.
- Journal
- Front Pharmacol
- Publish Year
- 2020
- Experiment Subject
- mouse; a549 cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Lung Fibrosis; Renal Fibrosis; Pulmonary Fibrosis; Alveolar Type Ii
- Paper Title Cn
- Paper Title En
- Arctigenin Suppressed Epithelial-Mesenchymal Transition Through Wnt3a/β-Catenin Pathway in PQ-Induced Pulmonary Fibrosis
- Bilingual Status
- semi_complete