ReferenceID 1130

Glycine suppresses kidney calcium oxalate crystal depositions via regulating urinary excretions of oxalate and citrate

J Cell Physiol

An abnormal urine composition is a key reason for kidney stone formation, but little is known about the roles of small metabolites in the urine during kidney stone formation. Here, we found urine glycine in patients with

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Reference Id
1130
Evidence Id
17720
Core Evidence Id
17720
Source Reference Id
2241
Herb2 Reference Id
HBREF003038
Subject Paper Key
HBIN015853_33772775
Pubmed Id
33772775
Doi
10.1002/jcp.30370
Paper Title
Glycine suppresses kidney calcium oxalate crystal depositions via regulating urinary excretions of oxalate and citrate
Paper Abstract
An abnormal urine composition is a key reason for kidney stone formation, but little is known about the roles of small metabolites in the urine during kidney stone formation. Here, we found urine glycine in patients with kidney calcium oxalate (CaOx) stone was significantly lower than that in healthy people via 1 H NMR spectra detection, and investigated the role and underlying mechanism of glycine in the regulation of CaOx stone formation. Our results showed that glycine could significantly attenuate ethylene glycol-induced CaOx crystal depositions in rat kidney via decreasing urine oxalate and increasing urine citrate. Mechanism studies revealed that glycine could decrease urine oxalate through downregulating Slc26a6 expression, whereas increase urine citrate via inhibiting Nadc1 expression. Moreover, glycine decreased the protein expression of both Slc26a6 and Nadc1 via increasing the expression of miRNA-411-3p, which directly bound to the 3'-untranslated regions of Slc26a6 and Nadc1 messenger RNAs, in vitro and in vivo. Together, our results revealed a novel role of glycine in the regulation of kidney CaOx crystal formation and provided a potential target for the treatment of kidney CaOx stone.
Journal
J Cell Physiol
Publish Year
2021
Experiment Subject
rat; patient; people
Experiment Type
Animal Experiment
Phenotype Related
Kidney Caox Stone
Paper Title Cn
Paper Title En
Glycine suppresses kidney calcium oxalate crystal depositions via regulating urinary excretions of oxalate and citrate
Bilingual Status
semi_complete