ReferenceID 1125
Tomatine Displays Antitumor Potential in In Vitro Models of Metastatic Melanoma
Int J Mol Sci
There is a growing interest in the cytotoxic effects of bioactive glycoalkaloids, such as alpha-tomatine on tumor cells. Here, for the first time, we determine the antitumor potential of tomatine, a mixture of alpha-toma
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 1125
- Evidence Id
- 17715
- Core Evidence Id
- 17715
- Source Reference Id
- 2227
- Herb2 Reference Id
- HBREF003024
- Subject Paper Key
- HBIN015731_32718103
- Pubmed Id
- 32718103
- Doi
- 10.3390/ijms21155243
- Paper Title
- Tomatine Displays Antitumor Potential in In Vitro Models of Metastatic Melanoma
- Paper Abstract
- There is a growing interest in the cytotoxic effects of bioactive glycoalkaloids, such as alpha-tomatine on tumor cells. Here, for the first time, we determine the antitumor potential of tomatine, a mixture of alpha-tomatine and dehydrotomatine, in metastatic melanoma (MM) cell lines harboring different BRAF and MC1R variants. We performed cytotoxicity experiments and annexin-V/propidium iodide staining to assess the apoptotic/necrotic status of the cells. ER stress and autophagy markers were revealed by Western Blot, whereas antiangiogenic and vascular-disrupting effects were evaluated through a capillary tube formation assay on matrigel and by ELISA kit for VEGF release determination. Cell invasion was determined by a Boyden chamber matrigel assay. Tomatine reduced 50% of cell viability and induced a concentration-dependent increase of apoptotic cells in the range of 0.5-1 muM in terms of alpha-tomatine. The extent of apoptosis was more than two-fold higher in V600BRAF-D184H/D184H MC1R cells than in BRAF wild-type cells and V600BRAF-MC1R wild-type cell lines. Additionally, tomatine increased the LC3I/II autophagy marker, p-eIF2alpha, and p-Erk1/2 levels in BRAF wild-type cells. Notably, tomatine strongly reduced cell invasion and melanoma-dependent angiogenesis by reducing VEGF release and tumor-stimulating effects on capillary tube formation. Collectively, our findings support tomatine as a potential antitumor agent in MM.
- Journal
- Int J Mol Sci
- Publish Year
- 2020
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Metastatic Melanoma; Tumor
- Paper Title Cn
- Paper Title En
- Tomatine Displays Antitumor Potential in In Vitro Models of Metastatic Melanoma
- Bilingual Status
- semi_complete