ReferenceID 1112
Diallyl trisulfide, a H2 S donor, inhibits cell growth of human papillary thyroid carcinoma KTC-1 cells through a positive feedback loop between H2 S and cystathionine-gamma-lyase
Phytother Res
Diallyl trisulfide (DATS), derived from garlic, is a well-known hydrogen sulfide (H2 S) donor. H2 S has recently emerged as a novel gasotransmitter involved in the regulation of cancer progression. The present study demo
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Record Fields
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- Reference Id
- 1112
- Evidence Id
- 17702
- Core Evidence Id
- 17702
- Source Reference Id
- 2204
- Herb2 Reference Id
- HBREF003001
- Subject Paper Key
- HBIN015205_31984539
- Pubmed Id
- 31984539
- Doi
- 10.1002/ptr.6586
- Paper Title
- Diallyl trisulfide, a H2 S donor, inhibits cell growth of human papillary thyroid carcinoma KTC-1 cells through a positive feedback loop between H2 S and cystathionine-gamma-lyase
- Paper Abstract
- Diallyl trisulfide (DATS), derived from garlic, is a well-known hydrogen sulfide (H2 S) donor. H2 S has recently emerged as a novel gasotransmitter involved in the regulation of cancer progression. The present study demonstrated that DATS along with other two H2 S donors, NaHS and GYY4137, significantly inhibited papillary thyroid carcinoma KTC-1 cells growth. DATS treatment triggered a rapid H2 S generation within 5 min in KTC-1 cells. Iodoacetamide, a potent thiol blocker reagent, partially rescued the cell membrane damage and ultimate cell death induced by DATS, indicating H2 S contributed to the apoptosis-inducing efficacy of DATS on thyroid cancer cells. Specifically, DATS treatment significantly upregulated the expression and enzymatic activity of cystathionine gamma-lyase (CTH), one of H2 S-producing enzymes, which was responsible for endogenous H2 S generation. After DATS treatment, H2 S quickly permeated cell membranes and activated NF-kappaBeta/p65 signaling pathway in KTC-1 cells. Nuclear translocated NF-kappaB bound to the promoter of CTH to enhance its transcription. These evidences proved that exogenous H2 S elevated CTH expression. CTH, in turn, catalytically generated a much higher level of endogenous H2 S. This positive feedback sustained excess H2 S production, which resulted in PTC cells growth inhibition. These findings may shed light on the development of novel H2 S-based antitumor agents.
- Journal
- Phytother Res
- Publish Year
- 2020
- Experiment Subject
- garlic; ktc-1 cells; papillary thyroid carcinoma ktc-1 cells; ptc cells
- Experiment Type
- Animal Experiment
- Phenotype Related
- Thyroid Cancer; Cancer; Papillary Thyroid Carcinoma
- Paper Title Cn
- Paper Title En
- Diallyl trisulfide, a H2 S donor, inhibits cell growth of human papillary thyroid carcinoma KTC-1 cells through a positive feedback loop between H2 S and cystathionine-gamma-lyase
- Bilingual Status
- semi_complete