ReferenceID 1106
Albiflorin attenuates high glucose-induced endothelial apoptosis via suppressing PARP1/NF-κB signaling pathway
Inflamm Res
Objective: Paeonia lactiflora Pall has long been recognized as an anti-inflammatory traditional Chinese herbal medicine. We aimed to study the pharmacological action of albiflorin, an active ingredient extracted from the
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Record Fields
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- Reference Id
- 1106
- Evidence Id
- 17696
- Core Evidence Id
- 17696
- Source Reference Id
- 2192
- Herb2 Reference Id
- HBREF002989
- Subject Paper Key
- HBIN015074_36357814
- Pubmed Id
- 36357814
- Doi
- 10.1007/s00011-022-01666-z
- Paper Title
- Albiflorin attenuates high glucose-induced endothelial apoptosis via suppressing PARP1/NF-κB signaling pathway
- Paper Abstract
- Objective: Paeonia lactiflora Pall has long been recognized as an anti-inflammatory traditional Chinese herbal medicine. We aimed to study the pharmacological action of albiflorin, an active ingredient extracted from the roots of Paeonia lactiflora Pall, on diabetic vascular complications. Methods: Human umbilical vein endothelial cells (HUVECs) were stimulated with high glucose and treated with 5, 10, and 20 μM albiflorin. CCK-8 assay, EdU staining, Annexin V-FITC staining, transwell assay, scratch test, RT-PCR, ELISA, Western blot, and immunofluorescence were carried out. SwissTargetPrediction database was used for screening targets of albiflorin and molecular docking was done using Autodock Vina software. Results: Albiflorin treatment dose-dependently alleviated high glucose-induced viability loss of HUVECs. In addition, albiflorin promoted the proliferation and migration, while inhibited apoptosis and the release of TNF-α, IL-6, and IL-1β in HUVECs. PARP1 was predicted and confirmed to be a target for albiflorin in vitro. Albiflorin targeted PARP1 to inhibit the activation of NF-κB. Transfection of HUVECs with PARP1 overexpression plasmids effectively reversed the effects of albiflorin on high glucose-treated HUVECs. Conclusions: Albiflorin suppressed high glucose-induced endothelial cell apoptosis and inflammation, suggesting its potential in treating diabetic vascular complications. The action of albiflorin possibly caused by its regulation on inhibiting PARP1/NF-κB signaling.
- Journal
- Inflamm Res
- Publish Year
- 2022
- Experiment Subject
- human; high glucose-treated huvecs; human umbilical vein endothelial cells; huvecs
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paeonia Lactiflora; Diabetic Vascular Complications
- Paper Title Cn
- Paper Title En
- Albiflorin attenuates high glucose-induced endothelial apoptosis via suppressing PARP1/NF-κB signaling pathway
- Bilingual Status
- semi_complete