ReferenceID 1043

Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo

Front Cell Dev Biol

Cardiac hypertrophy and fibrosis are major pathophysiologic disorders that lead to serious cardiovascular diseases (CVDs), such as heart failure and arrhythmia. It is well known that transforming growth factor beta (TGFb

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Reference Id
1043
Evidence Id
17633
Core Evidence Id
17633
Source Reference Id
2071
Herb2 Reference Id
HBREF002868
Subject Paper Key
HBIN008965_33117805
Pubmed Id
33117805
Doi
10.3389/fcell.2020.578197
Paper Title
Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo
Paper Abstract
Cardiac hypertrophy and fibrosis are major pathophysiologic disorders that lead to serious cardiovascular diseases (CVDs), such as heart failure and arrhythmia. It is well known that transforming growth factor beta (TGFbeta) signaling pathways play a major role in the proliferation of cardiac hypertrophy and fibrosis, which is mainly stimulated by angiotensin II (AgII). This study aimed to investigate the cardioprotective potential of isorhamnetin (ISO) in human amniotic epithelial stem cells (hAESCs) through global gene expression analysis and to confirm its beneficial effects on cardiac hypertrophy and fibrosis in the AgII-induced in vivo model. In vitro, biological processes including TGFbeta, collagen-related functions, and inflammatory processes were significantly suppressed in ISO pretreated hAESCs. In vivo, continuous AgII infusion using an osmotic pump induced significant pathological fibrosis and myocardial hypertrophy, which were remarkably suppressed by ISO pretreatment. ISO was found to reverse the enhanced TGFbeta and Collagen type I alpha 1 mRNA expression induced by AgII exposure, which causes cardiovascular remodeling in ventricular tissue. These findings indicate that ISO could be a potential agent against cardiac hypertrophy and fibrosis.
Journal
Front Cell Dev Biol
Publish Year
2020
Experiment Subject
human
Experiment Type
Cell Experiment
Phenotype Related
Heart Failure; Cardiovascular Diseases; Cardiac Hypertrophy; Arrhythmia; Cvds; Fibrosis; Myocardial Hypertrophy
Paper Title Cn
Paper Title En
Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo
Bilingual Status
semi_complete