ReferenceID 1029
Hydroxytyrosol decreases EDNRA expression through epigenetic modification in colorectal cancer cells
Pharmacol Res
The Mediterranean diet (MD) is one of the healthiest ones and is associated with a lower incidence of cardiovascular and cerebrovascular diseases as well as cancer. Extra virgin olive oil (EVOO) is probably the most idio
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Record Fields
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- Reference Id
- 1029
- Evidence Id
- 17619
- Core Evidence Id
- 17619
- Source Reference Id
- 2052
- Herb2 Reference Id
- HBREF002849
- Subject Paper Key
- HBIN007410_36528246
- Pubmed Id
- 36528246
- Doi
- 10.1016/j.phrs.2022.106612
- Paper Title
- Hydroxytyrosol decreases EDNRA expression through epigenetic modification in colorectal cancer cells
- Paper Abstract
- The Mediterranean diet (MD) is one of the healthiest ones and is associated with a lower incidence of cardiovascular and cerebrovascular diseases as well as cancer. Extra virgin olive oil (EVOO) is probably the most idiosyncratic component of this diet. EVOO has been attributed with many healthful effects, which may be due to its phenolic components, e.g. including hydroxytyrosol (HT). Recent studies suggest that EVOO and HT have molecular targets in human tissues and modulate epigenetic mechanisms. DNA methylation is one of the most studied epigenetic mechanisms and consists of the addition of a methyl group to the cytosines of the DNA chain. Given the purported health effects of EVOO (poly)phenols, we analyzed the changes induced by HT in DNA methylation, in a colorectal cancer cell line. Caco-2 cells were treated with HT for one week or with the demethylating agent 5'-azacytidine for 48 h. Global DNA methylation was assessed by ELISA. DNA bisulfitation was performed and Infinium Methylation EPIC BeadChips were used to analyze the specific methylation of CpG sites. We show an increase in global DNA methylation in Caco-2 cells after HT treatment, with a total of 32,141 differentially methylated (CpGs DMCpGs). Interestingly, our analyses revealed the endothelin receptor type A gene (EDNRA) as a possible molecular target of HT. In summary, we demonstrate that cellular supplementation with HT results in a specific methylome map and propose a potential gene target for HT.
- Journal
- Pharmacol Res
- Publish Year
- 2022
- Experiment Subject
- human; caco-2 cells; colorectal cancer cell line
- Experiment Type
- Cell Experiment
- Phenotype Related
- Cancer; Cardiovascular And Cerebrovascular Diseases; Colorectal Cancer
- Paper Title Cn
- Paper Title En
- Hydroxytyrosol decreases EDNRA expression through epigenetic modification in colorectal cancer cells
- Bilingual Status
- semi_complete