ReferenceID 1
Amplified cold transduction in native nociceptors by M-channel inhibition
J Neurosci
Topically applied camphor elicits a sensation of cool, but nothing is known about how it affects cold temperature sensing. We found that camphor sensitizes a subpopulation of menthol-sensitive native cutaneous nociceptor
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- Reference Id
- 1
- Evidence Id
- 16591
- Core Evidence Id
- 16591
- Source Reference Id
- 1
- Herb2 Reference Id
- HBREF000001
- Subject Paper Key
- HERB006931_24133266
- Pubmed Id
- 24133266
- Doi
- 10.1523/JNEUROSCI.1473-13.2013
- Paper Title
- Amplified cold transduction in native nociceptors by M-channel inhibition
- Paper Abstract
- Topically applied camphor elicits a sensation of cool, but nothing is known about how it affects cold temperature sensing. We found that camphor sensitizes a subpopulation of menthol-sensitive native cutaneous nociceptors in the mouse to cold, but desensitizes and partially blocks heterologously expressed TRPM8 (transient receptor potential cation channel subfamily M member 8). In contrast, camphor reduces potassium outward currents in cultured sensory neurons and, in cold nociceptors, the cold-sensitizing effects of camphor and menthol are additive. Using a membrane potential dye-based screening assay and heterologously expressed potassium channels, we found that the effects of camphor are mediated by inhibition of Kv7.2/3 channels subtypes that generate the M-current in neurons. In line with this finding, the specific M-current blocker XE991 reproduced the cold-sensitizing effect of camphor in nociceptors. However, the M-channel blocking effects of XE991 and camphor are not sufficient to initiate cold transduction but require a cold-activated inward current generated by TRPM8. The cold-sensitizing effects of XE991 and camphor are largest in high-threshold cold nociceptors. Low-threshold corneal cold thermoreceptors that express high levels of TRPM8 and lack potassium channels are not affected by camphor. We also found that menthol--like camphor--potently inhibits Kv7.2/3 channels. The apparent functional synergism arising from TRPM8 activation and M-current block can improve the effectiveness of topical coolants and cooling lotions, and may also enhance TRPM8-mediated analgesia.
- Journal
- J Neurosci
- Publish Year
- 2013
- Experiment Subject
- mouse
- Experiment Type
- Animal Experiment
- Phenotype Related
- Cold Temperature Sensing; Analgesia
- Paper Title Cn
- Paper Title En
- Amplified cold transduction in native nociceptors by M-channel inhibition
- Bilingual Status
- semi_complete