Clinical TrialID 180
In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines
NCT03728192
Apoptosis
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Record Fields
Scalar fields from the final clinical_trial record.
- Clinical Trial Id
- 180
- Evidence Id
- 180
- Core Evidence Id
- 180
- Source Clinical Trial Id
- 173
- Herb2 Clinical Trial Id
- HBCT000173
- Nct Id
- NCT03728192
- Title
- In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines
- Status
- Completed
- Phase
- Study Result
- No
- Study Condition
- Apoptosis
- Study Type
- Interventional
- Study Design
- Allocation: NON_RANDOMIZED|Intervention Model: PARALLEL|Masking: NONE|Primary Purpose: PREVENTION
- Intervention
- OTHER: mangosteen extract
- Intervention Allocation
- Non_Randomized
- Intervention Model
- Parallel
- Intervention Mask
- Intervention Purpose
- Prevention
- Gender
- All
- Age
- Child, Adult, Older_Adult
- Enrollment
- 2
- Outcome Measure
- apoptotic potential of ethanolic extract of mangosteen pericarp on oral and cervical cancer cell lines via apoptotic assay, Apoptosis assay was performed using Annexin V/FITC Kit (BD Biosciences, Catalog no. 556547), and the fluorescence intensities of FITC-conjugated annexin-V and Propidium iodide (PI) in cells were analyzed using flow cytometry. HeLa and H357 cells (1×106 cells/well) were seeded in a 6-well plate. The cells were allowed to adhere for 12 hrs, cultured in medium containing different concentrations of mangosteen extract for 48hr. The cells were then collected and washed twice with Phosphate buffer Saline, gently resuspended in 100μL annexinV-FITC binding buffer (1x) and incubated with 5μL annexinV-FITC in the dark for 10 min at 25°C. This was followed by centrifugation of cells at 2000 rpm for 5 min, and gently resuspended in 500μL annexinV-FITC binding buffer (1x) and 5μL PI was added in an ice bath, followed by immediate analysis by flow cytometry Cell Quest software (BD Biosciences)., 48 hrs at base line
- Sponsor Collaborator
- Meenakshi Ammal Dental College and Hospital
- Funded By
- OTHER
- Location
- Other Id
- MAHER-MU-004-IEC/2016
- Start Date
- 2016-03-07
- Primary Completion Date
- 2016-05-11
- Completion Date
- 2018-07-13
- First Posted
- 2018-11-02
- Results First Posted
- Last Update Posted
- 2018-11-02
- Study Document
- Study Url
- https://clinicaltrials.gov/study/NCT03728192
- Title Cn
- Title En
- In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines
- Bilingual Status
- semi_complete