Clinical TrialID 1218
To Exam the Effects of Phyllanthus Niruri Extracts on Human Neutrophils
NCT05206058
Neutrophil|Phyllanthus Abnormis Poisoning
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Record Fields
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- Clinical Trial Id
- 1218
- Evidence Id
- 1218
- Core Evidence Id
- 1218
- Source Clinical Trial Id
- 1201
- Herb2 Clinical Trial Id
- HBCT001201
- Nct Id
- NCT05206058
- Title
- To Exam the Effects of Phyllanthus Niruri Extracts on Human Neutrophils
- Status
- Completed
- Phase
- Study Result
- No
- Study Condition
- Neutrophil|Phyllanthus Abnormis Poisoning
- Study Type
- Observational
- Study Design
- Observational Model: |Time Perspective: p
- Intervention
- PROCEDURE: venipuncture
- Intervention Allocation
- Intervention Model
- Intervention Mask
- Intervention Purpose
- Gender
- All
- Age
- Adult
- Enrollment
- 240
- Outcome Measure
- Measurement of superoxide anion release, Superoxide anion released from human neutrophils were determined by measuring ferricytochrome c reduction.The neutrophils were activated with fMLF (30 nM) and cytochalasin B(0.5 μg/mL) (fMLF/CB). Changes in absorbance that occurred at 550 nm were observed continuously using a double beam spectrophotometer. Superoxide anion level was calculated using the methods described in previous report., After neutrophil isolation, an average of 3 months|Measurement of intracellular ROS formation, Neutrophil ROS production was determined from the conversion of non-fluorescent DHR 123 to fluorescent rhodamine 123, detected using flow cytometry. Neutrophils (2 × 106 cells/ml) were incubated with DHR 123 (2 μM) for 15 min at 37°C, and then treated with honokiol (0.1-10 μM) for 5 min before the addition of fMLP/CB (0.5 μg/ml) for a further 5 min. The change in fluorescence was analysed using flow cytometry, After neutrophil isolation, an average of 3 months
- Sponsor Collaborator
- Chang Gung Memorial Hospital
- Funded By
- OTHER
- Location
- Chang Gung Memorial Hospital, Taoyuan, 333, Taiwan
- Other Id
- 202002189B0
- Start Date
- 2022-01-01
- Primary Completion Date
- 2022-09-30
- Completion Date
- 2023-07-31
- First Posted
- 2022-01-25
- Results First Posted
- Last Update Posted
- 2023-09-01
- Study Document
- Study Url
- https://clinicaltrials.gov/study/NCT05206058
- Title Cn
- Title En
- To Exam the Effects of Phyllanthus Niruri Extracts on Human Neutrophils
- Bilingual Status
- semi_complete